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The transposase needed to remobilize the donor element is provided by a helper element (jumpstarter).
For stability of the new insertions, only individuals carrying a new insertion and lacking the helper element (i.e. DsRed negative) were chosen.
X-chromosomal insertions that were homozygous lethal or sterile could be obtained only if the following is considered: Because only new transformants that segregated away from the helper element were selected, hybrid females had to be used to set up P2 crosses in order to avoid bias against new X-linked insertions.
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These observations suggest that passive spreading via intact helper elements plays a role in rooA proliferation.
However, greater detail in the positive roles of Th2 responses in resolution of inflammation in cryptococcal infection and the timing of the requirements of the different T helper elements in still required.
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Table 1 Keywords used to describe mapping relationships Keywords Descriptions Keywords Descriptions Helper Mapping Rules between Elements Type Types of Mapping Relationships Mapper Mapping Rules between Attributes Query Mapping Rules between Attributes Key Elements or Attributes in the Objective Model Value Elements or Attributes in the Source Model Condition Preconditions Node Types of Elements.
Elements often have attributes or other elements, so the "helper" tag usually nests "helper" tags, "mapper" tags and "query" tags. 2.
Cationic liposomes were formulated with and without the targeting ligand folate conjugated to distearoylphosphatidyl ethanolamine polyethylene glycol 2000 DSPE-PEG20000), the novel cytofectin 3β[N N1,N1-dimethlaminopropylsuccinamidoethane -carbamoyl]cholesterol (SGO4), which coN N1,N1-dimethlaminopropylsuccinamidoethane -carbamoyl]cholesteroloylphosphatidylethaN N1,N1-dimethlaminopropylsuccinamidoethane -carbamoyl]cholesterol
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