This can help the pedagogue develop a plan for optimizing what is otherwise a haphazard process.
We thank John Leavitt for help optimizing the muconic acid assay methods and Allison Wagman for help performing the assays.
WRF would like to thank J. Maitin-Shepard for help optimizing the Python scripts, D. Zerbino and M. Schulz for numerous tips and bug corrections to Velvet and Oases during the process of assembly, C. Dunn and M. Howison for preliminary versions of the Agalma package.
Leuven) for their help with the functional assays and isolation of primary rat hepatocytes, Dr. Bipasha Bose (SCIL) for help with optimizing the immunofluorescence stainings and Tineke Notelaers (SCIL) for help with differentiation and functional studies.
We are grateful to Scott Basehore and Yves Konigshofer for help in optimizing purification and detection steps, respectively.
We thank Dr. James Fraser and Dr. Tom Lisney for help in optimizing the ERG experimental apparatus and procedures, Dr. William Nelson and Dr. Nikolaus Troje for suggestions regarding modeling procedures, Dr. Chris Hofmann for providing lens transmission data for cichlids, and Dr. Karen Carleton for qPCR primer sequences and helpful suggestions for gene expression analysis.
We would like to thank Dr. S Meyer and D. White University off Manchester) for their help in optimizing the culture conditions of the MUTZ-3 cell line and L. Vantomme and S. Baute for the excellent technical assistance.
It is hoped that the results of this study would help in optimizing substituents for better binding at enzyme pockets and guide in the design of more effective HIV-PI of this class.
Furthermore, a systematic investigation of the impact of the different thresholds used here, including thresholds on posterior probabilities and size of CMBs, would help in optimizing the thresholds for specific applications more objectively.
The ideal of equality may therefore help us optimizing health care for the entire population.
