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The HELP assay has been combined with massively parallel sequencing (HELP-Seq) and/or array-based platforms [ 25, 26].
Furthermore, it also can help assay some given diseases [1, 2, 11].
Recently, Leschenko et al. have reported the global methylation of a series of primary MCL using the HELP assay [23].
To detect global patterns of epigenetic changes that could distinguish between IUGR and controls, we performed the HELP assay on the CD34+ cells.
We employed our new, high-resolution version of the microarray-based HELP assay [33] to study ∼1.32 million loci throughout the human genome.
We therefore proceeded to bisulphite MassArray experiments [34], initially testing the technical performance of the HELP assays by choosing four loci, two each representing constitutively hypomethylated and constitutively methylated sites identified by the HELP assay.
Similar(30)
High-resolution HELP assays were performed according to recent advances in the technology [33].
Recent studies have described genome wide methylation in ALL using different approaches such as differential methylation hybridization [24], CGP-island arrays [25], [26], [27], MCA/human proximal promoter DNA microarray [14] or HELP assays [28].
Anti-DNP antibodies in the serum of immunized mice or in the culture supernatants of help assays were measured by ELISA.
For in vitro help assays, cells from spleens were depleted of B cells, other MHC class II+ cells and CD8+ T cells to purify CD4+ T cells.
For help assays, mice were injected either with 200 µg alum-precipitated DNP-KLH along with 10 killed Bordetella pertussis bacteria (Lee laboratories, Grayson, USA) or 150 µg alum-precipitated OVA with 10 B. pertussis bacteria.
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