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In order to determine whether AML and ALL samples display distinct DNA methylation signatures, we performed unsupervised clustering of HELP array data.
The HELP array experiments were performed using biological replicates.
Gene expression microarray data and the HELP array data are available at the Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/geo/) (Accession number GSE39895).
Information about the positions of probes, M-values obtained from different samples and k-means cluster number are described in Supplementary Table S2, and gff files of the HELP array data are available at our web site (http://www.brc.riken.go.jp/lab/mcd/mcd2/protocol/nanoHELP.html).jp/lab/mcd/mcd2/protocol/nanoHELP.html
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The personnel of the MicroCribi Microarray Service (http://microcribi.cribi.unipd.it), Chiara Romualdi and Francesco Martinati are kindly acknowledged for help in array data analysis.
In addition, due to the uniform signal and distinct pattern produced by the hph oligos, they help align array matrices with images, avoiding data collection errors due to mis-alignment.
The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1755-8794/5/52/prepub The authors would like to thank Kai-Tai Zhang, Department of Etiology and Carcinogenesis of Peking Union Medical College as the help of array CGH experiment.
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But in general, in older children who do not look very distressed, fever is positive evidence of an active immune system, revved up and helping an array of immunological processes work more effectively.
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