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For quantitative growth and developmental assays (apical extension rate and aerial hyphae height), mutants that exhibited a ±5 mm/d difference relative to wild type were considered significant (Colot et al. 2006; Park et al. 2011b).
The best-known examples are the gibberellic acid (GA -insensitive Rht-1 and GA -insensitive-1 dwarfing genes that were essentiandto the Green Revolutions in wheat and rice, respectively (Peng et al. 1999; Monna et al. 2002; Sasaki et al. 2002), but height mutants have also been widwarfingd in other cereals.
The peak heights of those two sites were measured and compared with the corresponding heights of mutant and wild-type peaks present at the heteroplasmy site.
In this table we also included the relative peak height of the mutant peaks compared to the wild type peak as observed in the mutation assays.
Wild type to mutant height ratio was also measured.
Obviously, the reduction of plant height for the mutant is completely attributed to the shorter culm length.
This gene has been shown to affect plant height, when comparing mutant and wild type alleles [ 52].
This was simply the average height of the mutant plants divided by the average height of the wild type plants [ 3, 14].
Five tall mutants were found in DongnongV7 variety, and the average height of the mutants was 31% taller than that of the control.
It was previously assumed that the reduction in plant height of sub mutants was at least in part due to stem twisting.
Single T-DNA insertion mutants, prf1-4 and prf2-1, showed very similar phenotypic effects, with plants showing defects in normal rosette leaf morphology as well as inflorescence development, leading to shorter overall plant height for these mutants (Fig. 1).
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