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The hME primers for each multiplex reaction were spotted onto SpectroCHIPs using the Sequenom CCU RoboDesign nanodipenser, detected on the Sequenom MALDI-TOF mass spectrophotometer (MS), and adjusted to normalize MALDI-TOF MS peak height intensities for subsequent multiplexing reactions.
The carbonyl index was calculated from the height intensities at a wavenumber of 1732 cm−1 corresponding to the C=O vibration, normalised to the maximum height of the amide I band at 1620 cm−1.
However, a more detailed analysis by calculating the amide III crystalline index using the relative height intensities from the bands at wavenumbers of 1260 and 1227 cm−1 reveals subtle changes in the fibre conformation (Fig. 5; ESM Fig. S 3).
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This software identifies with 99% accuracy heterozygous nucleotide positions, by employing an algorithm that incorporates measures of peak height, peak intensity, and background noise from ABI electrophereograms.
Additional data: Tephra fall volume (bulk, DRE) Ignimbrite volume (bulk, DRE) Intra-caldera deposit volume (bulk, DRE) Column height Intensity Magma type.
The peak height intensity ratio (I 335/I 333) of the peak of 335 nm to the peak of 333 nm was plotted against the logarithm of polymer concentration.
The Raman height intensity ratios of the peaks I2935/2880, I2844/2880 and I1090/1130 are representative of the interaction of (1) with the alkyl chains and furnish information about the ratio between disorder and order that exists in the conformation of the alkyl chain.
Canopy height, intensity, and density metrics characterizing the canopy structure were calculated at a cell resolution of 20 m across the entire LiDAR collection, along with topographic metrics from the DTM resampled to the same 20-m x 20-m (400 m2) cells [29].
The defined peak clusters were then quantified and compared using either peak height (intensity) or peak area (trapezoidal rule) as outlined in the Results and Discussion.
The resulting MS chromatogram should pass the following criteria: The retention time has to be 7.5 min ± 15%; in the total ion current chromatogram, the baseline level intensity should be ≤4.5 e6 with the noise intensity ≤7.5 e5; for the extracted ion chromatogram (m/z 365 ± 0.5), the peak height intensity ≥4.5 e6 with a peak width at half height of ≤55 s.
Canopy height, intensity, and density metrics characterizing the canopy structure were calculated at a cell resolution of 20 m across the entire LiDAR collection, along with topographic metrics from the DTM resampled to the same 20-m x 20-m (400 m) cells [ 29].
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