Sentence examples for heavy label from inspiring English sources

Once confirmed, PSD proteins on the list can be quantitatively evaluated in conjunction with immunoprecipitation and heavy label internal standards.

For 2 out of 3 biological replicates, mock samples were labeled with "light" and Myc samples with "heavy" label, whereas labels were swapped for the third replicate.

Topograph deconvolutes the isotopologue distributions to determine the amount of heavy label in the amino acid precursor pool (Fig. S2 and S5) and from this, correctly calculates the percentage of peptides that are newly synthesized (18).

Carbamidomethylation of cysteines was set as fixed modification and oxidation of methionine; dimethyl "light" label (K and N-term) and dimethyl "heavy" label (K and N-term) were set as a dynamic modifications.

Although we measured newly synthesized proteins by heavy label incorporation, as old mouse body weights were not significantly changed during the last 7 weeks of the experiment and young weights changed only 1% per week (Fig. S1), we believe that protein synthesis and degradation are essentially in equilibrium at this time.

For SILAC labeling (Ong et al, 2002) of human cells, medium was supplemented with unlabeled amino acids (light label), with arginine:HCl, 13C6 (Cambridge Isotope Laboratories), and lysine:HCl, 4,4,5,5-d4 (Sigma) for the medium label, or with arginine:HCl 13C6, 15N4 and lysine:HCl, 13C6, 15N2 (Sigma) for the heavy label.

Akt1 expressing HEK 293 cells were cultured in light, medium and heavy labeled SILAC media.

Akt1 expressing HEK293 cells were cultured in light and heavy labeled SILAC media.

For heavy labeling, 4 µL of formaldehyde-D2 (4% in D2O) was added.

Stable heavy isotope labeling of HK-2 cells was achieved using [13 C6]-Lys (heavy) labeling combined with exposure to 500 μM UA.

Normal lysine and arginine were incorporated as light labels while for heavy labeling the isotopes were 8 and 10 Da heavier.