Sentence examples for heated subjected from inspiring English sources

Lysates normalized for protein content (Bradford protein assay, Bio-Rad) were prepared in Laemmli buffer, heated, subjected to SDS-PAGE on 10% acrylamide gels and transferred onto nitrocellulose membranes.

In response to questions, Mr. O'Rourke took direct issue with Governor Cuomo on three often heated subjects - the death penalty, legislation banning discrimination against homosexuals and the use of Medicaid funds for abortions.

Diversity at the leadership level is a bit of a heated subject.

Jump to media player Australia celebrates its national day on 26 January but it's becoming an increasingly heated subject.

These two plates are symmetrically heated and subjected to a constant heat flux.

Both cytoplasmic and nuclear proteins were mixed with SDS buffer, heated and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE).

The entire solution methodology is illustrated in detail on the particular examples of the plate fin heat sink subjected non-uniform heat density on the surfaces.

Two rice genotypes differing in heat tolerance were subjected to heat stress of 40 °C for 2 h after flowering.

Heated samples were subjected to 4 12% SDS PAGE, transferred to nitrocellulose, and immunoblotted with Ac-p53 (K382) antibody (Cell Signaling Technologies).

Equal amounts of total proteins for each sample were submitted to 37°c heating and subjected to SDS/PAGE (12%).

What was really not expected was the fact that those populations lost part of their heat tolerance, being no more subjected to heat stress.