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Try to allow your attorneys to handle whatever you can and if things get too heated, step away.
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Numbers beside solid and open circles show the corresponding temperature (°C) of each heating step.
The pTRM checks were performed at every second heating step to detect magnetomineralogical changes produced by heating throughout the paleointensity experiment.
The initial increase in the kinetic energy is related to the heating step of the system; subsequently, it is kept constant during the simulation.
Fig. 6 Elastic stresses in 22-direction at the end of the heating step for three different thicknesses of bond coat and tbc.
The induced magnetization on a heating step increases at about 370 °C, reached a maximum at 500 °C, and decreases from 500 °C to 570 °C.
After heating step, 5 mL trichloroacetic acid (10%%) was added and centrifuged the mixture at 1000 rpm for 10 min at 4 °C.
The first weight loss occurs during the heating step from 50 to 290 °C (32 %), which is due to the dehydration and decomposition of nitrates.
In order to increase yields, a heating step of 10 min at 70°C was performed before the mechanical lysis step of the manufacturer's protocol.
These results clearly demonstrate that the heating step we introduced efficiently retrieves the antigens for immunostaining on cryosections without losing or damaging samples.
Even though the heating step we introduced sounds drastic, it was rather gentle and preserved organ and tissue morphology both at embryonic and juvenile stages.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com