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Male adult rats were anaesthetized (ketamine, 60 mg/kg, and medetomidine, 0.25 mg/kg), the cervical region of their backs were shaved and cleansed with iodine and core temperature was maintained close to 37 °C using a self-regulating heated blanket.
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Body temperature was monitored and controlled using a rectal temperature probe and heating blanket, respectively.
A heating blanket was used to maintain rectal temperature at 36.5°C to 37.5°C.
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The arm used for blood sampling was put under a heating blanket at 55°C to obtain arterialized venous blood.
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