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Paraffin sections of human OSCC were subjected to heat-mediated antigen retrieval (citrate buffer, pH6) prior to blocking.
Unmasking of the antigen retrieval was performed by heat-mediated antigen retrieval method in 10 mm citric acid (pH 6.0).
HspB2 is localized in the mitochondria and protects cells against heat-mediated cell demise [41].
Wild-type and Atxn2 ko mice responded similarly to heat-mediated painful stimuli.
Immunohistochemistry was performed using the heat-mediated antigen retrieval method with citrate buffer.
Thereafter, sections were subjected to 3-min heat-mediated antigen retrieval.
Immunohistochemical staining was carried out on 4-μm paraffin sections after heat-mediated antigen retrieval.
This involves a cyclophilin-mediated modification of R/G opsin, possibly involving proline isomerization.
The NBS mediated modification of purified enzyme resulted in total loss of activity.
Ubiquitin-mediated modification plays a critical role in regulating protein stability (Ciechanover and Schwartz, 1994).
Enzyme-mediated modification sites on numerous substrates could be revealed by large-scale proteomic analysis of enriched peptides.
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