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Heat stock in saucepan over medium heat.
In a separate pan, heat stock to boiling.
Transfer to a platter. 5. Heat stock to barely simmering.
Heat stock with ginger and garlic to a simmer.
Purified pET21a harboring the lysin genes of interest, were transformed into E. coli BL21 (DE3) (Invitrogen, Carlsbad, CA) by heat stock at 42°C for 30 s. BL21 (DE3) transformants were cultured at 37°C in 1 L modified LB (mLB) medium (15 g/l tryptone, 8 g/l yeast extract, 5 g/l NaCl) supplemented with 150 μg/ml ampicillin.
Clones were induced by a single heat stock of 45 min at 37°C, adult midguts were analysed at 7, 14, or 21 days after clone induction (ACI), and at least 10 clones/midgut were examined in n = 10 adult midguts.
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anthropogenic heat stocks at region β [J].
In August 2004, the 23 wards of Tokyo generated an anthropogenic heat stocks of 2106.5 TJ/day.
Also presented is the concept of Heat Island Integral, which measures the difference of anthropogenic heat stocks between two regions.
Also presented is a concept of Heat Island Integral, which measures the difference of anthropogenic heat stocks between two regions.
The problem of how to represent anthropogenic heat stocks in any block was analyzed in Sato (2006) who introduced the concept of Heat Island Integral.
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