Sentence examples for heat fragmentation from inspiring English sources

The heat fragmentation of mRNA is incorporated and combined with the denature step of 1st strand cDNA synthesis in the presence of Mg contained in the 1st strand cDNA buffer.

The mRNA then underwent a mild heat fragmentation followed by random priming for first-strand synthesis.

The purified mRNA was then subjected to a mild heat fragmentation followed by random priming for first-strand synthesis.

A sensible performance degradation that reduced the overall storage capacity of about 50% has been noticed after 20 cycles, presumably due to local powder heating, fragmentation and subsequent compaction.

HCD fragmentation exhibits cleavages closer to basic residues than CAD, which is consistent with CAD being a slower heating fragmentation process that allows protons slightly more time to migrate toward the center of TMDs prior to cleavage.

Heat during fragmentation is avoidable with Covaris acoustic shearing.

Heat-induced fragmentation of RNA introduces mostly random breaks into the phosphodiester backbone.

The mRNA was randomly fragmented by heating to 94°C in fragmentation buffer.

Nascent RNA was first fragmented using Fragmentation reagents (AM8740; Ambion, Carlsbad, California) by heating at 70°C for 5 min. Fragmented nascent RNA were then purified and used for standard Illumina library preparation.

The results of ASTEC applications against the following experiments are presented: FARO (corium jet fragmentation), LIVE (heat transfer between a molten pool and the vessel), MASCA (separation and stratification of corium non miscible phases) and OLHF (mechanical failure of the vessel).

The biotinylated cRNA products were then fragmented to 50 200 bp prior to hybridization by heating cRNA in 1× fragmentation buffer (40 mM Tris-acetate, pH.8.1, 100 mM potassium acetate, 30 mM magnesium acetate) at 95°C for 35 min. Published sequences from cDNA libraries were obtained from the TIGR catfish gene index release 5.0 [ 19].