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To address the origin of the shift in Ca2+ sensitivity in failing hearts, cells were treated with PKA after incubation with PKCα and ε.
In neonatal mTert-GFP transgenic hearts, cells coexpressing mTert-GFP and Sca-1 were observed (Fig. 6 A ) and quantified by fluorescence-activated cell sorting (FACS) analysis.
As in the uninjured hearts, cells lacking the expression of all investigated antigens were identified in the injury zone and in the periphery of the injury (Fig. 5 F).
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For instance, they developed into monkey heart cells and nerve cells.
"The last thing we want to do is grow rogue heart cells," Dr. Marbán said.
In normal mice, only 1percentto3percentcent of the heart cells near the injury could divide.
In the MRL mice, up to 20percentt of the heart cells divided in response to injury.
The damage from a heart attack is permanent because heart cells cannot regenerate the way liver cells can.
The hope is that the cells might be turned into heart cells, brain cells or other types of cells to repair damaged and diseased organs.
But instead of forming scar tissue, the mice generated new cells that were indistinguishable from neighboring healthy heart cells.
Looking at heart cells, one shaman, an elderly woman, asked the pathologist to show her a "happy heart".
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