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The histamine uptake by the isolated guinea-pig heart was measured using a rapid dual-isotope dilution method [ 45] and Langendorff's technique for the isolated heart with constant perfusion flow.
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The area, length, width and width/length ratio of ~100 CMs per heart were measured using the public domain Java image processing program ImageJ [ 40], as described previously [ 11].
BAX protein levels in MI and treated hearts were measured using Western blotting.
BAX mRNA levels in MI and treated hearts were measured using RT-PCR.
Chewing gum as well as mood and well-being measures was the same as those used in Studies 2 and 3. Heart rate was measured using Polar s610 heart rate monitors with Spectra 360 gel.
The heart rate was measured using a pulse oximeter.
Heart rate was measured using a PPG100C amplifier and a photoplethysmograph transducer.
Heart rate was measured using a telemetric system (Polar Vantage NV, Polar Electro KY, Kempele, Finland).
Heart rate was measured using POLAR pulse monitors (Käyttö 4000 RUD 11.93 STK model, with readings every 15 sec).
ATP concentration in heart tissue was measured using luciferase-based ATP determination kit (Invitrogen, Paisley, UK) according to the manufacturer's instructions.
After a minimum of 15 20 min acclimatisation in a temperature controlled room (21 ± 2 °C) systemic blood pressure and heart rate was measured using a digital sphygmomanometer (UA-767, A&D Instruments Ltd., UK).
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