The heart tissue was dissected by slowly pipetting every 15 min.
Brain, heart, kidney, liver, intestine, dorsal root ganglial tissues were dissected, perfused with phosphate-buffered saline to remove blood, blotted with Kim-wipes to remove excess buffer, and immediately freeze-clamped at the temperature of liquid nitrogen.
Tissues were dissected on ice in the following order: liver, heart, brain, and white skeletal muscle.
Forty-eight hours after injection (n = 3), the animals were euthanized and tissues were dissected from 6 different organs: liver, spleen, kidney, lung, heart, and brain.
6, 24, or 48 hours after injection (n = 3) the animals were euthanized and tissues were dissected from twelve different organs: kidney, spleen, liver, colon, brain, heart, lung, pancreas, muscle, small intestine, gonads, and bone marrow.
Subcutaneous tissues were dissected bluntly.
Cortical and medullary kidney tissues were dissected and prepared separately.
Lung tissues were dissected six days after PQ injection.
Multiple tissues were dissected from the mice.
Tissues were dissected in cold sterile insect saline.
