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Heart tissues were collected from transgenic mice and homogenized with cold lysis buffer (25 mM Tris HCl [pH 7.6], 10 mM NaCl, 1.0 mM EDTA, 2.0 mM MgCl2, 0.5 mM DTT, and protease inhibitor).
BALB/c mice were infected with 103 TCIdosedofe of CVB3 for various time periods or with different CVB3 doses for 4 days, the heart tissues were collected, homogenized and then subjected to ELISA assays.
Heart tissues were collected and analyzed for markers of oxidative stress, UPS activation, inflammation and autophagy.
At 11 days post-norepinephrine administration, mice were sacrificed and heart tissues were collected.
Neonatal heart tissues were collected and fixed in 4% cold glutaraldehyde, postfixed using 1% osmium oxide, and progressively dehydrated using alcohol.
E17.5 heart tissues were collected from Snrk wild-type (WT), KO and HET embryos, and the proteins were isolated using homogenization in RIPA buffer (Sigma) with complete mini ETDA-free protease inhibitor cocktail (Roche) and PhosStop phosphatase inhibitor (Roche) using a Qiagen TissueRuptor.
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Heart tissue was collected from transgenic mice and extracts were prepared by homogenization with the extraction buffer [25 mM Tris-HCl pH 7.6, 400 mM NaCl, 0.5% NP40, 5 mM EDTA, 25% Sucrose, 2 mM PMSF and Protease inhibitor (Sigma Inc., Catalog P-834040)].
When field conditions allowed, blood samples were collected; otherwise, heart tissue was collected.
In brief, after different treatments, the heart tissue was collected and homogenized in 50 mM ice-cold potassium phosphate buffer (pH 6.8).
After fixation, liver, heart, and eye tissue were collected and paraffin-embedded.
Thereafter, the tumor, spleen, liver, kidney, spleen, heart, and lung tissues were collected to perform Prussian blue Fe staining.
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