Cardiac function and heart tissue were analyzed at 6 weeks post MI.
Survival of heart tissue was analyzed by histology and macroscopically by the presence of visible beating for grafts under the renal capsule.
The following normal tissues were analyzed: heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes.
Although several tissues were analyzed (spleen, skeletal muscle, esophagus, ileum, colon and heart muscle), the microscopic findings of T. cruzi-induced damage in DNA immunized and infected dogs were limited exclusively to heart tissue.
The survival rates, systolic blood pressure (sBP), heart rate (HR), peripheral oxyhemoglobin saturation (SpO2), and shock index were monitored for 60 min, and lung tissues were analyzed histopathologically.
DNA samples obtained from frozen brain tissues were analyzed by nested PCR using several primer pairs.
Stained tissues were analyzed using confocal microscopy.
Lung tissues were analyzed using Affymetrix GeneChip microarrays.
WT control tissues were analyzed in parallel.
In order to investigate the first two questions, (clock) gene expression patterns of several organs (i.e., adrenal, liver, pineal, heart and muscle tissue) were analyzed after light exposure at circadian time (CT) 14 and CT20.
