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Total RNA was extracted from heart tissue samples using Trizol Reagent (Invitrogen, CA, U.S ., according to the manufacturer's instructions.
Genomic DNA was extracted from the heart tissue samples using a QIA amp DNA Mini Kit according to manufacturer's instructions (Qiagen, Hilden, Germany).
Total RNA was extracted from cell populations containing CSCs, from human iPSCs, and raw human heart tissue samples using the RNeasy Plus Mini Kit (QIAGEN) as positive/negative control.
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The Affymetrix RAE230A and RAE230 2.0 microarray chips were used for heart tissue samples and PBMC respectively.
Study design: Heart tissue samples from 110 explants were analysed for PVB19 using primers and a 5′-nuclease probe designed to amplify a 160-basepair PCR product from the VP1/NS1 gene region.
Liver and heart tissue samples were analyzed for non-heme iron content using the bathophenanthroline method as described by Torrance and Bothwell [ 58].
Heart tissue samples ranging from 30 to 120 mg were used for RNA extraction.
Heart tissue samples were taken from nine of the SIDS victims and eight from the control group.
Moreover, selective NET binding in in vitro autoradiography was observed in human brain and rat heart tissue samples.
High activity was mainly observed from the tumor and heart tissue samples (Fig. 3E).
Some heart tissue samples (4 months and 24 months) were obtained from the Aged Rodent Tissue Bank at NIA.
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