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The embryogenesis process ceases at the late heart stage in red1-1 atd athehe bent-cotyledon stage in red1-2, resultingely, resulting in seed abortion in both lines.
The Robot Heart stage, on the other hand, pushed a genre that could be dubbed "Playa house" all night long.
Alright, alright, we're talking about Diplo joining Skrillex on the Robot Heart stage, where his decision to drop "Turn Down For What" was allegedly met with a roar of disapproval.
The Mothership was on par with stages at any premiere festival in the world, the Robot Heart stage brought Playa vibes to the desert expanse in droves, and the multi-leveled, whitewashed Booba Cosmica stage off in the distance was one of the most unique stage designs I've ever seen and the sound at all three stages was pristine.
By comparison, in corresponding wildtype siliques, 94% of the embryos were at heart stage.
In mutant siliques at the heart stage (majority, 54%, of embryos at heart stage), a relatively large number of embryos remained in the pre-globular (18%) and globular (18%) stages of development.
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In the wild-type, GUS activity is barely detectable during the globular-heart stage transition (Fig. 3A), and then resolving to the procambial traces in the pro-cotyledons, hypocotyl and root of older embryos (Fig. 3B).
Among 166 ESTs detected (49 from apical cells, 117 from basal cells), fourteen were not detected in heart-stage embryos (four from apical cells, ten from basal cells).
To trace the fate of the transcripts identified from apical or basal cells in the subsequent development stages, hundreds of transcripts from the two cDNA libraries were examined in global-stage proembryos and heart-stage embryos.
Two families of auxin-regulated genes (the Aux/IAA, and GH3 families) have been identified among the transcripts that were specifically found in 16 DAP heart-stage embryos.
GSE7511, which contains 23 samples to identify all genes active in 10 different compartments, was isolated using the Leica AS LMD system from heart-stage seed.
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