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The clinical records, imaging studies, and heart specimens of 22 consecutive patients with DI-DORV were reviewed.
Heart specimens were immediately collected post-mortem and analysed for nitrotyrosine formation and expression of the endogenous H2S-producing enzyme cystathionine γ-lyase (CSE).
In order to determine if the presence of SB, MB and APM could be related with the degree of development of the tricuspid valve, we reviewed 32 postnatal and 26 fetal human heart specimens with tricuspid atresia (TA).
In contrast, the mutant with DbpB overproduction was not recovered from any of the heart specimens within the first 5 weeks post-inoculation, but it was indeed grown from some heart specimens between 6 weeks and 4 months of infection.
Heart specimens were fixed for 24 h in 10% formalin, then dehydrated in graded ethanols and embedded into paraffin blocks.
Although the mutant disseminated to most of the joint, remote skin and heart specimens at 2 weeks, majority of ear tissues were not colonized until 3 weeks.
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Augmented nitrotyrosine formation has also been demonstrated in the heart specimen of septic patients [37, 38].
Fig. 1 Nitrotyrosine staining of heart specimen collected at the end of the experiment after 24 h. a Two representative histological images of sham (left) and septic (right) animals (×10 magnification).
We report herein a unique case of cystic tumor of atrioventricular (AV) node (CTAVN), which, to our knowledge, is the first of its kind diagnosed in an explanted heart specimen and only the fourth diagnosed antemortem.
Sham n = 5, sepsis n = 8 animals Fig. 2 CSE (cystathionine γ-lyase) staining of the heart specimen collected at the end of the experiment after 24 h. a Two representative histological images of sham (left) and septic (right) animals (×10 magnification).
As shown in Table 1, the dbpAB mutant modified with dbpA overexpression was recovered from each heart specimen from all of the 24 mice that had received a dose of 102 or 103 organisms, albeit the five infected mice in the lowest dose group did not produce a positive heart specimen.
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