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Transmitted light images of heart sections were processed using DP2-BSW software (Olympus Corp).
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Sections were processed as previously described [23].
Sections were processed for immunohistochemistry as follows.
Cortex and striatal sections were processed as described [58].
Briefly, sections were processed in parallel using identical antibody concentrations.
All sections were processed under the same standardized conditions.
Biotinylated sections were processed as described before [10].
Floating sections were processed for immunostaining as described previously [7].
Free-floating sections were processed for immunohistochemistry as described previously.
Then the sections were processed for DAB visualization.
Sections were processed with hematoxylin and eosin (HE) staining.
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