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For the analysis of cross sectional cell sizes, standard HE-stained heart sections were mounted with VectaShield mounting medium (Vector Laboratories), images were taken with a Keyence BZ digital microscope (Keyence, Germany), and cross-sectional areas of myocytes displaying round-shaped nuclei were quantified using the ImageJ software (NIH).
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Hearts were fixed in 4% formaldehyde, imbedded in paraffin, and 4 μm sections were mounted on SuperFrost Plus glass slides (Menzel-Gläser, Baunschweig, Germany).
Sections were mounted with DPX.
Sections were mounted in mowiol for imaging.
Sections were mounted with Permount (Fisher Scientific, Pittsburgh, PA).
Sections were mounted with Vectashield containing DAPI.
Sections were mounted using Hydromount (National Diagnostics).
Sections were mounted in SlowFade mounting medium (InVitrogen).
Sections were mounted in Aquamount.
Sections were mounted using Aquamount.
Sections were mounted in Gelmount.
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