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Fetal heart sections were also stained with anti-OTR antibody (Fig. 1J) and with anti-OT antibody the staining was barely visible (Fig. 1K).
For morphological staining, heart sections were also stained with hematoxylin and eosin or Masson's trichrome reagent.
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Paraffin sections were also cut from block samples of the esophagus, stomach, small intestine, colon, heart, lung, thyroid, liver, pancreas, kidney, adrenal gland and urinary bladder.
Sections were also stained by immunofluorescence.
Images of the heart sections were captured.
Frozen heart sections were stained with anti-MF20 and DAPI.
Heart sections were deparaffinized, rehydrated, and washed in distilled water.
Heart sections were stained using Masson Trichrome (IMEB, San Marcos, California).
Two heart sections were scored for each animal.
The heart sections were prepared using standard pathological procedures.
For autoradiography of the heart, the transplanted hearts were sectioned at 30 μm thickness using microtome and heart sections were placed on glass slides.
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