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Haematoxylin and eosin staining was performed to assess the cross-sectional area of cardiomyocytes in heart sections.
The histological examinations of the heart sections are shown in Fig. 3.
The degree of fibrosis in heart sections was assessed by Masson's trichrome staining.
These short axial heart sections were washed with PBS and stained with mouse monoclonal anti-TNC mouse 4F10TT, the same antibody used for autoradiography, with direct immunoperoxidase technique.
Serial short-axis heart sections 20 μm thick were obtained by sectioning on a cryostat to create a series of rings for autoradiography.
Serial short-axis heart sections of 20 μm thick were obtained using a cryostat to create a series of rings for autoradiography.
The distribution profile of infiltrated mast cell-subpopulations and eosinophils in the lung and heart sections of the patients who died of severe allergic hyperresponsiveness, was investigated.
Unfixed mouse heart sections were immersed at room temperature in 100% precooled Acetone for 10 minutes.
Aorta and heart sections were analyzed by haematoxylin-eosin (HE) and oil red O-staining.
Finally, signs of fibrosis and calcification were not observed in dt heart sections (Fig. 7).
Ki67 positive nuclei were immunostained in 20 µm thick heart sections.
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