The numbers of human heart samples used for qRT-PCR for each genotype were given under each column.
It is important to point out that the heart samples used in the present investigation are taken from patients with various cardiovascular diseases and that the sample size is rather small.
The performance of the transcription was tested in different heart sound samples using real-time and non-real-time processes.
Total RNA was extracted from heart tissue samples using Trizol Reagent (Invitrogen, CA, U.S ., according to the manufacturer's instructions.
Genomic DNA was extracted from the heart tissue samples using a QIA amp DNA Mini Kit according to manufacturer's instructions (Qiagen, Hilden, Germany).
Total RNA was extracted from cell populations containing CSCs, from human iPSCs, and raw human heart tissue samples using the RNeasy Plus Mini Kit (QIAGEN) as positive/negative control.
Recent attempts at direct isolation of Francisella spp. from environmental samples using cysteine heart agar with 9% chocolatized sheep blood have yielded three strains (TX07-6608, TX07-7308, and TX07-7310) from seawater collected in the Galveston Bay area, Texas [ 13].
Five to ten μg of RNA from heart samples was used for miRNA microarray.
