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In an affinity chromatography application one bed was derivatized with Cibacron Blue 3GA and used for the purification of lactate dehydrogenase from a crude bovine heart extract.
Natural substances from animals are, for example, amniotic fluid, aorta extract, Bombyx lipida, brain extract¸ bubulum oil (oil from the feet of cattle), equus extract, heart extract, liver extract, marrow extract, maromota oil, or serum.
Finally, high binding affinity and specificity of the peptide ligand are confirmed by the purification of t-PA from crude porcine heart extract using the immobilized-ligand column for affinity chromatography.
There were insufficient amounts of heart extract to analyze by Western blot.
As previously reported [5], U1013 anti-mXin antibody recognizes a major band mXinα (155 kDa) and its splicing variant mXinα-a (250 kDa) as well as a 340 kDa mXinβ band in mouse heart extract (Fig. 4).
In brief, the strains were inoculated in basal medium (trypticase 1g/dl, yeast extract 0.3 g/dl, heart extract 0,3 g/dl, NaCl 0.2 g/dl, L-cysteine-HCl 0.03 g/dl, agar 0.1 g/dl, Tween 80 0.025 ml/dl, pH 7.0) and in basal medium supplemented with 1g /dl of each of the carbohydrates ribose, sorbitol, erythritol, and glucose (positive control).
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High performance liquid chromatography of newborn rat heart extracts indicated that OT-GKR was a dominant form of OT.
In agreement with this hypothesis, bacterially expressed DSG2 ICS was able to bind PKP2 from COS-1 cells, whereas COS-1 cell-derived DSG2 ICS did not bind to PKP2 from rat heart extracts (Online Supplement Figure 2).
For this purpose, and to avoid tissue specificity, we subjected mixture of RNA samples from four tissues (brain, lungs, muscle, and heart) extracted from a single C. chamaeleon specimen to MPS using the SOLiD ABI platform.
It is not known if acute pulmonary zinc exposure inhibits cardiac aconitase activity; however, zinc transfer from metal-lothionein to cardiac mitochondrial aconitase has been noted in mouse heart extracts (Feng et al. 2005).
At the end of the procedure (50 min), the animals were immediately terminated by cervical dislocation and the heart extracted and ventricular tissue snap frozen in liquid nitrogen and stored at −80°C.
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