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Head disengagement might be promoted during this reaction by a conformational change that Pds5-Wapl imposes.
Head disengagement, although essential, is not the limiting step during loading.
We next analysed whether we could detect Smc head disengagement when Scc1 is cleaved and the cohesin ring dissociates from chromosomes in anaphase.
(Our finding that ATP hydrolysis during unloading can be replaced by prolonged incubation without nucleotide reflects an alternative, probably unphysiological, way to achieve head disengagement).
This suggests that ATP hydrolysis promotes an early step during DNA exit from the cohesin ring that can in part be replaced by a longer incubation without nucleotide, possibly corresponding to SMC head disengagement.
Similar(7)
The committee can help maximize the effectiveness of each individual board member and address issues of disengagement or dysfunction head on.
The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome.
Both reactions therefore likely involve disengagement of the SMC ATPase heads.
Beyond the Manchester response and the upcoming election, the British government, heading as it is toward "Brexit" and disengagement from the European Union, faces important decisions about how to deal with security challenges.
The two complexes might catalyze their respective part of a concerted loading reaction that requires disengagement of both the ATPase heads and the Rad21N-Psm3 interface.
Gen. Giora Eiland, a career soldier who recently relinquished his Army position to become head of the government's National Security Council, will head the team working on the disengagement plan, Mr. Sharon's office said in a statement.
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