The sense sequence of Hes-1 primers was 5'-AGAAGAGGCGAAGGGCAAGA-3′, whereas the antisense sequence was 5′-CGGAGGTGCTTCACAGTCAT-3′.
He kept primer and gunpowder in his garage, which he used to reload ammunition of various sizes.
Before he wrote "Primer," which won the grand jury prize this year at Sundance, he worked as a software engineer.
The pLenti6/V5-D-FLAG Hes-6 vector was constructed by cloning a PCR-amplified fragment of Hes-6 into the EcoRI site of pcDNA3-FLAG and then amplifying the FLAG-tagged Hes-6 with primers flanked by SpeI sites for cloning into the SpeI site of pLenti6/V5-D.
When Mr. Stanley, who lives in Scottsdale, Ariz., thought about buying a home in Latin America, he decided he needed a primer.
In an interview in 1984, Geisel pulled off the shelf "The Riverside Reader," a dusty, dog-eared primer he used in 1909 when he was in the first grade.
It's typical ABC fare: a beige primer of "he said, she said" that makes no firm ground in any direction.
And so in 1954, when John Hersey published a piece in Life deploring the books used in public schools to teach children how to read — he called them "pallid primers [with] abnormally courteous, unnaturally clean boys and girls" — he mentioned Dr. Seuss as one of the "imaginative geniuses" whom publishers might turn to in the hope of enriching the books they produced for the schools.
Several requests, he said, concerned DNA primers, the snippets of DNA used to extract specific genes from an organism.
As a primer, he suggested "Cape Bulbs," by Richard L. Doutt (Timber Press, 1994; $35).
