In addition, all 31 bovine (and ovine/caprine) syntenic groups have been definitively assigned to specific chromosomes on the basis of official marker assignments performed with both G/Q and R-banded chromosomes of cattle (Hayes et al. 2000), as well as in those of both sheep and goat chromosomes (Di Meo et al. 2003).
Flash chromatography on silica gel (0 25% EtOAc/toluene, linear gradient) separated the two regioisomeric, mono-alkylated products (3 and 4; The regiochemistry was not definitively assigned).
It is true that the politics of offence are used to shut down dissident voices of all kinds, frequently in minority communities, and the understanding of culture as a type of property to which ownership can be definitively assigned is, at the very least, problematic.
The ants from Tilin amber are one of the earliest records of crown-group ants, as only two Cretaceous species have been definitively assigned to extant subfamilies until now: one formicine from New Jersey Turonian amber and one dolichoderine from Canadian Campanian amber23,24.
13C NMR spectra were definitively assigned with reference to HSQC correlation spectra (not presented).
The concentration and size of each PCR product were verified on agarose gels; gene identity was definitively assigned following the sequencing of 300 base pairs (bp) of each PCR product.
Fifty samples could not be definitively assigned to a known HAdV type due to insufficient amplified DNA for sequencing, the presence of a mixed virus infection that could not be resolved or, in a few cases, to significant differences between the sequenced strain and known reference sequences.
Therefore, the strain sequences for the virus in these patients could not be definitively assigned (category unresolved, Table 3).
Acquiring spectra at 5 °C resulted in sharpening of some spectral features, but overall, resonances could not be definitively assigned.
However, the lower-frequency band at 2543 cm 1 could not be definitively assigned to cysteine because there is still positive intensity near 2543 cm 1 in D2O.
Specific reservoirs of the etiologic agent cannot be definitively assigned; however, we have cultivated M. ulcerans from a single aquatic insect from Benin (10 ).
