Sentence examples for hci buffer from inspiring English sources
A 50 μL aliquot of cell lysate (as prepared for DNA quantification assay) was added to a 96-well plate along with 50 mL of the alkaline phosphatase substrate (p-nitrophenyl phosphate in diethanolamine HCI buffer, pH 9.8) and shaken gently, and the absorbance was measured at wavelengths of 405 and 620 nm using a ELx800 microplate colorimeter (BioTek Instruments Inc).
The incubation solution was 170 mM Tris-HCI buffer (pH 8.2) containing 1% bovine serum albumin, bacitracin (40 μg/ml) and MgCl2 (10 mM) to inhibit endogenous proteases.
For each case, a control slide was incubated with Tris-HCI buffer substituted for the primary antibody.
The cells were thoroughly washed with PBS and lysed with 0.1% Triton-X 100 in 100 mM Tris-HCI buffer, pH 7.4.
Color visualization was performed with liquid DAB chromogen in imidazole-HCI buffer (pH 7.5) containing hydrogen peroxide until the brown color fully developed.
For this, 100 μL of enzyme extract was mixed with 1.89 mL of 50 mM Tris-HCI buffer, pH 7.6, containing 10 mM KCN.
The reaction mixture contained 0. 3 ml Tris-HCI buffer, 0.1 ml NADP, 0.1 ml glucose-6-phosphate, 0.1 ml MgCI2, 0.02 ml supernatant and 2.38 ml of H2O.
Sections (4 μm) mounted on poly- L-lysine-coated slide were incubated for 30 min at 60°C, deparaffinised by standard methods, and placed in 0.05 M Tris-HCI buffer, pH 7.2.
The TK1 enzyme extract was prepared as follows: The tissue was homogenized in a Teflon homogenizer with two volumes of 0.05 M Tris-HCI buffer, pH 8.0, containing 0.34 M sucrose, 25 mM KCI, 5 mM MgCI2, 7 mM EDTA, 0.5% NP-40, and 2 mM 4-2 aminoethyl benzenesulfonyl fluoride hydrochloride (Pefabloc, Fluka, Seelze, Switzerland).
