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Total C (TC, dry combustion), inorganic C (IC, modified pressure calcimeter), and HC (extraction at 80 °C) were measured and organic C (OC) was calculated by difference (TC − IC).
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HC participated in RNA extraction and cDNA preparation.
In addition, six gender and age-matched healthy controls (HC) (TNP-48.8±7.5 yrs; HC-49.0±8.1 yrs) were recently included without history of chronic pain (e.g. migraine) or recent surgery in the trigeminal region (e.g., third molar extraction).
First-in-human studies with healthy controls (HC) found a low first-pass extraction resulting in low uptake of [18F]GE-180 in healthy tissue [10, 11].
MiRs from sera of patients with treatment naïve early RA (ERA), with treated established RA and HC were isolated by phenol-chloroform extraction.
The efficacy of this test was compared with a reference PCR-ELISA, using MY09/11 and GP5+/6+ consensus primers and the use of a single extraction method for both HC-II and PCR-ELISA assays was validated.
Twenty fresh specimens from each of three pairs of hydrologically-connected populations were sampled for mRNA extractions: 1) SCS and OC, 2) HC and US, and 3) MDS and FRC.
XHX, HC, and JPM performed molecular experiments (material collection, RNA extraction, real-time PCR).
The Mediterranean Sea is exposed to a high risk of pollution by petroleum hydrocarbons (HC), due to the presence of tens of sites related to their extraction, refinery, and transport along its coastline [ 1].
MI performed cell wall preparations, protein extraction and separation, as well as preparation of samples for MS. HC analyzed all the MALDI-TOF MS spectra.
Following 6 hr incubation with 100 μg/mL pooled APS-IgG or HC-IgG, total RNA was isolated from HTR-8 cells using phenol chloroform extraction.
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