Sentence examples for hc binding from inspiring English sources

For comparison, HC metabolism was analyzed with a more mechanistic model that accounted for HC binding to HSA, in which only unbound HC was metabolized by hepatocytes.

The amplified cDNAs were first purified with Purelink PCR Purification Kit (Invitrogen, HC Binding Buffer) and eluted in 100 μL of EB (Invitrogen).

After tagmentation, a Purelink PCR Purification Kit with HC Binding Buffer was used for purification and eluted with 30μL of EB or H2O.

Biochemical studies indicate an increase in the number of presynaptic high-affinity choline transporter (HACT) sites, as assessed by [3H]hemicholinium-3 (HC-3) binding.

ML352 exhibited noncompetitive choline uptake inhibition in intact cells and synaptosomes and reduced the apparent density of hemicholinium-3 (HC-3) binding sites in membrane assays, suggesting allosteric transporter interactions.

HC-3 binding showed a substantial increase during the course of nicotine treatment and again, the effect was limited to the midbrain.

Although the cerebral cortex showed little or no change in HC-3 binding during or after nicotine administration, activity was reduced persistently in the hippocampus.

Autoradiographic distribution of HC-3 binding sites was evaluated in the hippocampus of the offspring at postnatal days 15, 25, and 53.

DOI: http://dx.doi.org/10.7554/eLife.05560.006 We next used RNA interference (RNAi) to examine a role for Insigs in the 25-HC-induced binding of reductase to UBIAD1.

First, we observed that ML352 demonstrated dose-dependent inhibition of HC-3 binding, well-fit by a single-site inhibition model (r = 0.948) with a Ki of 128.6 ± 15.3 nM, similar to that found in uptake inhibition studies.

HC-3 binding capacity (Bmax) and affinity (Kd) and ML352 Ki values were determined using nonlinear curve fit to a single-site inhibition model using Prism 5. ML352 was tested in the Lead Profiling Screen (Eurofin, Luxembourg), a binding assay panel of 68 G-protein coupled receptors, ion channels, and transporters, at 10 μM.