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Finally we amplified exons 6 13 of CYP3A43 and found a splice variant having deletion of exons 7 and 8 and partial inclusion of 121bp of intron 7.
One of them (having deletion of both the 7 and 8 exons and an insertion of 121bp of intron 7; Fig 8e) would generate a complete open reading frame.
RT-PCR amplification of exons 1 5 generated a product of the expected size (432bp; Fig 8b), however, amplification of exons 6 10 generated two splice variants having deletion of exon 7 (149bp) or exon 8 (128bp, Fig 8c).
The result suggests that there is a concomitant deletion of TERT in the majority of participants having deletion of 5p.
A sample was considered having deletion of 1p locus when more than 70% of counted nuclei exhibited one target signal (red) and two reference signals (green).
We included this tool for finding consensus motifs [ 39] as there is a possibility of having deletion or insertion (indels) in the binding sites of the peak sequences from ChIP-Seq.
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Interestingly, expression of Ey ∆HD, which has deletion of the HD domain, significantly increased binding of Ey to Ey2 binding site while expression of Ey ∆PD, which has deletion of the PD, did not.
CMA results indicate that, compared with the reference Lymphoid, only Erythroid has deletion in this region.
with MDS/AML, three had the T354M mutation, and one had deletion T355.
One patient RB15 had deletion of four bases which was also detected in his father.
Mesenchymal GBMs have deletion of NF1, mutation of TP53 and PTEN, and loss of CDKN2A.
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