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New versions of the vectors which have unique sites for insertion of HomA, HomB and HomC were therefore made.
All the plasmids have unique sites for the restriction enzyme AlwN1 ~0.8 1.2 kb away from the gene regions in the vector DNA, which was used for the secondary digestion of the MNase-digested naked DNA or chromatin for the IEL analysis.
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This subcloning step is necessary to have the insert of BAC2 in a vector which has unique sites (in this case I- PpoI) positioned adjacent to the two regions of homology to be used for the next recombination step.
Although most acute care hospitals had unique site codes, LTCFs historically had reported to I-NEDSS through local health departments without a site code.
pLoxPuro and pLoxBsr have unique NheI sites inside of the mutant loxP-flanked region.
pLoxNeo, pLoxPuro and pLoxBsr have unique SpeI sites outside of the mutant loxP-flanked region.
The vectors pBACLinkSp and pBACLinkGm have been designed with ease of cloning in mind and have unique restriction sites for cloning the homology regions.
Fetuin-A is also known to have unique apatite-binding sites ([94]; reviewed also by Jahnen-Dechent et al. in ref. [75]).
Furthermore, an alternative model of transcription in Drosophila proposed that four major blocks of genes, coded on the same strand, have unique transcription initiation sites upstream of their coding region [56], [57] This implies that transcription of one coding region is possible [8].
The previous research indicated that remote construction sites have unique problems that need unique and customized IT solutions.
In the Kingdom of Saudi Arabia, remote construction sites have unique problems and these have been highlighted by Justanyah and Sidawi (2011, Sidawi 2010a,b, 2012a,b.
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