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These chunks have to be sequenced many times to find identifiable overlaps so that they can be pieced together, says Gustavo Stolovitzky, the head of the functional genomics and systems biology group at IBM, a computer giant.
Mutations in more than 200 different genes are known to cause many of these disorders and in less obvious disorders several genes may have to be sequenced in order to identify the correct diagnosis in a patient.
Namely, almost all of the RIL individuals have to be sequenced to detect useful QTLs.
But since for MLST seven house-keeping genes have to be sequenced it is expensive and time-consuming.
However, because of a high % of rRNA reads, ENC libraries have to be sequenced deeper to obtain sufficient transcriptome coverage.
Also, the generation of suitable insert-specific sequencing primers can require a huge effort when large numbers of clones have to be sequenced, each with multiple primers.
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So only a small part of the genome has to be sequenced directly.
These processes introduce errors and mean that a gene has to be sequenced several times to ensure a reliable result.
DEFOG almost tripled the size of the initial repertoire of human ORs in a single experiment, and only 7% of the PCR clones had to be sequenced.
In order to fully characterize a human transcriptome, millions of transcript tags had to be sequenced.
Also, such hairpins have made sequencing very challenging: constructs containing the mirrors had to be sequenced piecemeal, as the sequencing reaction would stop at the very edges of the hairpin.
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