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A decoy set was used if the sequence of the experimentally solved structure is the same as the sequence given initially in the CASP competition, so that the majority of decoy structures have the same sequence as the native structure.
We used only sets in which the decoy structures have the same sequence as the native structure, and that contain the coordinates of all the heavy atoms (see Table S1).
The resistant progenies IR78221-19-6-7-B, IR78221-19-6-99-B, and IR78221-19-6-99-B have the sequenceuence as Moroberekan while the susceptible progeny IR78221-19-3-196-B hasequenceme sequence as Vandana.
For all four OXO genes, the resistant progenies, IR78221-19-6-7-B, IR78221-19-6-99-B and IR78221-19-6-99-B, have the sequenceuence as Moroberekan, while the susceptible progeny, IR78221-19-3-196-B, hasequenceme sequence as Vandana.
A no-RT control was also used to ensure that there was no contaminating DNA in the RT reaction; this is important because H2B genes do not contain introns and so have the same sequence as cDNA.
Quantification of the BoNT product peptides is achieved by using stable isotope-labeled internal standards that have the same sequence as the native product peptides but are labeled with C. Figure 3 shows typical HPLC-ESI/MS/MS chromatograms obtained during the quantification of the activity of BoNT-A, and a standard curve for both of the product peptides.
Similar(54)
After cDNA sequencing, the 31 groups were reduced to 17 because some EcoTILLING groups had the same sequence, as was previously detected for the eIF4E gen. These false positive rates were due to weak bands that were taken into account to minimise the haplotype loss.
First, all 21 O. rufipogon accessions with Ping have only Ping16G, which has the same sequence at + 16G/A as mPing (Table 1).
The primers for miRNAs have the same sequences as Gallus gallus miRNAs with an appropriate adjustment at their 5′ terminus.
To confirm the deduced chemical structure of the mass shift, we synthesized a peptide that has the same sequence as the in vivo H2B peptide and contains a benzoyl group at H2BK5.
The mechanism can be harnessed to silence genes in plants by expressing self-complementary single-stranded (hairpin) RNA in which the duplexed region has the same sequence as part of the target gene's mRNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com