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Currently, we have successfully expressed several bioactive recombinant proteins using the BmBacPAK6 expression system and B. mori pupae as a bioreactor.
We have successfully expressed glycosylated, biologically active IAG using the Pichia pastoris yeast expression system.
In summary, we have successfully expressed the endoglucanase Spr Cel8A from S. proteamaculans in E. coli.
Here, we have successfully expressed recombinant human bikunin (rh-bikunin) in Pichia pastoris and also established the purification procedure.
Using this vector system, we have successfully expressed enhanced green fluorescent protein (EGFP) and firefly luciferase (Luc) in G. frondosa.
We have successfully expressed HFB1 from Trichoderma reesei belonged to class II of hydrophobins in Pichia pastoris.
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While RP expression in other green microalgae has not been as thoroughly explored as in Chlamydomonas reinhardtii, Chlorella ellipsoidea has successfully expressed therapeutic recombinant proteins including mature rabbit neutrophil peptide 1 (NP-1) for innate immune system defense and flounder growth hormone for aquaculture (Rasala and Mayfield 2015).
Zhou and his coworkers had successfully expressed NS1 in Pichia pastoris and obtained 70 mg/L [ 24].
After the transfection, the RNA extracts were submitted for RT-PCR, cloning and sequencing to check whether the expressed RNA encompasses both the vector part and the inserted sequence, an evidence that the transfected sequence has successfully expressed in our system.
Our laboratory has successfully expressed a variety of recombinant proteins in soybean seeds, including subunit vaccines for traditional injection and oral delivery [ 9, 11, 12], immunogens for treatment of autoimmune disease, and diagnostic reagents for the detection of cancer [ 5, 8].
Smooth and non-muscle myosins have been successfully expressed in non-muscle expression systems [17], and might be expected to fold to a considerable extent in the reticulocyte lysate as well.
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