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We chose this configuration because in previous experiments we have observed efficient MGMT-based selection at the stem cell level using the SFFV promoter [19], [33], and we have also observed efficient EGFP expression using the PGK promoter in both myeloid and lymphoid lineages [14], [34].
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We have used this line to generate a liver-specific knock-out of the frataxin gene [13], and have observed a unambiguously efficient rate of recombination in our initial study, i.e. all animals ever analyzed within the initial study group were bona fide knock-outs.
"I have observed for myself that when I feel that I need to work a lot, I get much less efficient," she says.
I have observed you.
I have observed your children.
Having observed the highly efficient and powerfully simplifying nature of the radical-mediated dimerization strategy in the construction of C3 C3′ vicinal quaternary centers, we wished to extend this transform to the full spectrum of dimeric cyclotryptamine and diketopiperazine alkaloids.
- has observed.
"I've observed that.
They've observed what works.
Resellers are seeing the same thing that we've observed.
It has been observed that efficient individual cellulase activities do not necessarily translate into good hydrolysis on a substrate like lignocellulosic waste, e.g. rice straw or wheat straw.
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