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As expected the majority of pseudogenes have no reads aligning to their sequence.
Large dark boxes 1−3 indicate regions of SN15 scaffold 2 that have no reads mapped from Sn4 and Sn79 and are likely to be absent from the newly sequenced strains.
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Among these, 420 transcripts have no read mapped from root and shoot data sets, indicating their expression in mature leaf and/or flower buds (Supplementary Table S4).
This yielded summarized read counts for 36,229 genes annotated by Ensembl 59. 12,632 of these genes had no reads across all lanes and were not considered further.
Fundamentally, pre-fetchable memory space has no read "side effects," which means no matter how many times one reads it, one gets the same result.
If true, and I've no read to believe it's not true, I imagine E3 this year will be all about Halo.
Another 7,414 genes had no reads for at least one B6 and one D2 sample and were considered to be unreliable.
One gene (DEFB6) has no reads aligned 30 DPP and an average of 28 reads aligned 15 DPP.
Alignments in intergenic positions were sometimes located close to genes that had no reads mapping within them.
TWIST2 had no reads in MDCK cells and too few in MDCK-Ras cells to perform differential expression analyses.
Overall ~97% of the annotated genes had more than one read mapped, while merely ~3% of the annotated genes had no reads mapped, indicating good sequencing depth.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com