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Furthermore, one gene can have multiple essential requirements during an organism life cycle, precluding investigation of all but the first essential requirement in progeny homozygous for a non-conditional mutation.
The mammalian Aurora members A, B and C are serine/threonine protein kinases that have multiple essential roles during mitosis.
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This protein has multiple essential roles in the replication cycle involving RNA binding, viroplasm formation, helicase and can hydrolyse the γ-phosphate of RNA and NTPs acting as an RTPase and NTPase.
The CXCR4 protein has multiple essential functions, including homing of stem cells and metastasis of cancer cells (Miki et al, 2007).
S100 proteins have multiple and essential functions in many different tissues, mainly in cells of epithelial origin.
Temperature-sensitive alleles of essential genes are powerful genetic tools, as many essential genes have multiple requirements throughout the life of an organism: with TS alleles, one can bypass early requirements at the permissive temperature and study later requirements after up-shifts to the restrictive temperature (O'Rourke et al. 2011a).
Being a public figure makes it more essential to have multiple accounts, according to several executives.
According to our statistics, only ∼1% of the E. coli essential genes have multiple copies in S. enterica.
22 Nevertheless, because one single miRNA might have multiple targets, judicious considerations are essential for identification of the main functional target.
Furthermore, in order to identify statistically significant changes in metabolite concentrations in the order of 20 to 100%, it was essential to have multiple replicates.
A detailed preclinical investigation of the mechanism of action of drugs such as E7080, which have multiple kinase targets, is therefore essential to maximize its potential clinical application.
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