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This approach is only feasible in species for which there have been EST sequencing projects.
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Second, there must have been ESTs expressed in tumours for the candidate genes in the Unigene database.
In recent years there have been extensive EST-based gene discovery programmes for parasitic nematodes.
Accession numbers correspond to protein sequences, except when less than three EST sequences have been found, where EST accession numbers are been given.
Subsequent additions of cotton ESTs to Genbank (> 210,000 ESTs) have been compiled into a large EST assembly [TIGR Cotton Gene Index 8, (CGI8)] [ 11].
Only the Phytophthora infestans, Phytophthora sojae, Pythium ultimum and Saprolegnia parasitica EST databases have been searched, as the EST database of the Phytophthora ramorum genome was not available.
The P. bellina flower bud EST sequences have been submitted to the EST database with the accession numbers CK857580-CK859399 and CO742089-CO742627.
However, to date, only a limited number of SSR markers have been developed from EST databases for blueberry.
Transcriptional maps with single nucleotide polymorphism (SNP) markers have been published using EST information.
Previous gene expression studies have been performed using EST sequencing, spotted microarrays and Affymetrix GeneChip technology.
Five hundred and seven new primer pairs have been developed from EST sequences containing microsatellite motives.
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