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For comparison, 40 mutations in which in vitro activities have been already assayed were also analyzed (see Supporting Information: Text S2, Table S2, Figure S1, Figure S2).
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Many commercially available assays have been already approved by FDA or have been clinically validated in accordance with international approved validation guidelines.
This multiplex assay is composed of 7 consecutive PCR reactions combining primers that have been already described in different studies [ 23, 25, 26] (Table 3).
The detailed procedure to test the reproducibility of the DASL assay has been already published by our group [ 14] (The specific details are noted in the Additional file 3 of the above mentioned published article).
As reference lines, they are amenable to repeated phenotypic measurements, and already a large collection of metabolic traits have been assayed.
These already large fractions may be underestimates, as only a subset of cell states have been assayed.
"There already have been".
Deals already have been announced.
There already have been others.
There already have been four.
Although several strains with reduced genomes have already been constructed, there have been no reports of strains constructed with deletions assayed for influence on growth.
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