If these complexes were sorbed at all, the resulting [F]sorbed was too low and could not be detected by the electrode which had a quantification limit of 2.6 μM.
In addition, we have added a quantification of the percentage of cells DHR123 (ROS) positive.
We have added a quantification of the overlap between an ER marker calreticulin and Mit-TEER in the text (Results, last paragraph).
Furthermore, as recommended by the reviewer, we have incorporated a quantification of the % SAbgal+/nGFP+ cells after co-implantation of control (nGFP+) and senescent (nGFP-) cells.
For Sema3A, though the gradient of endogenous Sema3A expression has previously been characterized in developing cortical tissues4, it is still difficult to have an exact quantification of the Sema3A concentration profile in a living mammalian brain.
As proposed, we have now included a quantification of the co-localization between Jen1 and the most relevant of these two markers, i.e. Sec7, in the gga1Δ gga2Δ mutant vs. WT cells (now displayed in Figure 4I).
We have also included a quantification of the activation energy for Hsp90 homologs (yHsp90 and Grp94) in Figure 4 figure supplement 2, utilizing previously reported rates found in Frey et al 2007.
We have now included a quantification of average α in regions of high EGFR-mCitrine intensity as a function of mean fluorescence that shows that increasing the RE-biogenesis by ectopic Rab11a-BFP expression increases the interaction of EGFR with the PTP1B trapping mutant.
Although these reports and others have calculated disease burden related to poor water supply, sanitation, and hygiene there has not been a quantification of the improvement in health related outcomes due to improvements in water supply and sanitation.
However, in order to articulate their model they have to rely on a quantification of the psyche, which undercuts precisely the premise, based on which psychophysical parallelism was considered a good alternative to psychophysical causality to begin with (Rickert 1900, 76; Rickert 1934b, 66 73).
