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Leaves from each week containing eggs were incubated separately to allow hatching of larvae.
The hatching of larvae occurred approximately 2 h after egg inoculation.
Likewise, embryos from crosses between five lamprey species vary in the stages at which fatality occurs, ranging from four cells, through to the hatching of larvae.
Since chitin is known to be present in the eggshell [ 57] and the microfilarial sheath [ 58] of nematodes, it has been suggested that chitinases have a role in remodeling processes during the molting of filariae and in the hatching of larvae from the eggshell [ 59, 60].
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Embryonic and larval viability (number of eggs hatching and of larvae reaching pupation) is comparable for tsh knockdown and controls (73% versus 75% hatch and 66% versus 64% reach pupation, n=900 and 600, respectively).
It is clear that the hatching of infective/juvenile larvae from embryonated eggs and the migration of second- or third-stage larvae (L2-L3) thostgh hostissueses are critical steps triggering infection and pathogenesis by T. canis.
The hatching of young larvae is achieved in several ways.
This will most probably lead to earlier phyto-and zooplankton peaks and possibly hatching of fish larvae.
Hatching of J2 larvae was stimulated by soaking the cysts in sterile 3 mM ZnCl2.
The cysts were soaked in 3 mM ZnCl2 as stimulus for hatching of J2 larvae under sterile conditions.
If the incubator water is too warm, the unsynchronized development and enzyme production may cause premature hatching of weak larvae.
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