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The calorimetric data reveals the presence of two sequential nature of binding sites where the first binding site has stronger affinity (~ 104 M− 1) and second binding site has weaker affinity (~ 103 M− 1).
The latter study shows FGF18 has weaker affinity for each receptor than FGF8b.
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Although not classified as a pure opioid, tramadol has weak affinity for μ-receptors and is also thought to interact with noradrenergic and serotonergic systems.
While VEGF121 has weak affinity to NRP1 [ 45], we assume it does not bind NRP1, and this does not change our results [ 43].
Since the TR has weak affinity for RGTTCA but recognizes RGGTCA motifs [ 20], these DR4 elements represent optimal TR binding sites.
In addition, although protein A and protein G have strong binding affinity for human IgGs, protein G has moderate affinity and protein A has weak affinity for mouse IgGs commonly used in research.
ConA also has weak affinity for complex type N-glycans, which we would expect to be present on the liver- and kidney-derived hGGT1, but under the conditions of our assay, no binding was observed on hGGT1 in these sample pools.
Polycyclic aromatic compounds have weaker affinity with four receptors.
Hence, the PAS of BChE had weaker affinity than AChE, which mediates substrate activation.
The mAb b12 has similar binding affinity to the gp120 monomer and trimer on the surface of infected cells [ 42], while those CD4bs-specific mAbs with no neutralizing activity have weaker affinity to the gp120/gp41 trimer.
HAs have weak affinity and are multispecies specific.
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