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While RP expression in other green microalgae has not been as thoroughly explored as in Chlamydomonas reinhardtii, Chlorella ellipsoidea has successfully expressed therapeutic recombinant proteins including mature rabbit neutrophil peptide 1 (NP-1) for innate immune system defense and flounder growth hormone for aquaculture (Rasala and Mayfield 2015).
Our laboratory has successfully expressed a variety of recombinant proteins in soybean seeds, including subunit vaccines for traditional injection and oral delivery [ 9, 11, 12], immunogens for treatment of autoimmune disease, and diagnostic reagents for the detection of cancer [ 5, 8].
After the transfection, the RNA extracts were submitted for RT-PCR, cloning and sequencing to check whether the expressed RNA encompasses both the vector part and the inserted sequence, an evidence that the transfected sequence has successfully expressed in our system.
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Currently, we have successfully expressed several bioactive recombinant proteins using the BmBacPAK6 expression system and B. mori pupae as a bioreactor.
We have successfully expressed glycosylated, biologically active IAG using the Pichia pastoris yeast expression system.
In summary, we have successfully expressed the endoglucanase Spr Cel8A from S. proteamaculans in E. coli.
Here, we have successfully expressed recombinant human bikunin (rh-bikunin) in Pichia pastoris and also established the purification procedure.
Using this vector system, we have successfully expressed enhanced green fluorescent protein (EGFP) and firefly luciferase (Luc) in G. frondosa.
We have successfully expressed HFB1 from Trichoderma reesei belonged to class II of hydrophobins in Pichia pastoris.
To that end we have successfully expressed the hydrosoluble ecto-domain of bovine CD38 (bCD38; residues 32 278), and corresponding glycosylation mutants, in the methylotrophic yeast Pichia pastoris.
In this study, using pBEV, we have successfully expressed and purified multiple human kinases produced in E. coli and insect cells.
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has successfully addressed
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has successfully extended
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has well expressed
has successfully persuaded
has successfully passed
has successfully processed
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