We have not amplified our message for gene array analysis in order to avoid PCR-mediated bias.
This scenario is supported by the observation that in other internally deleted elements (that have not amplified), the size distribution does not exhibit any strong bias (not shown).
Thus, despite having very similar structural zinc finger domains and intron-exon boundary organization, Bassoon zinc finger domains have not amplified in number in teleosts.
Linum was selected for the KAPA3G Plant PCR Kit evaluation as it had amplified and sequenced with Taq polymerase, while Anethum was chosen because it had amplified but failed to sequence, and Senna as it had not amplified at all.
Areas of six representative B. algerae clones from the Genomiphi-amplified DNA library were reamplified by PCR from genomic DNA to confirm that the Genomiphi process had not amplified chimeric sequences.
Three of these clades, containing human ADAM10, ADAM17 (Fig. 2) and MMP21 (Fig. 4), had not amplified on the vertebrate lineage and maintained a ciona to human gene ratio of 1 1.
Criteria for retesting were as follows: cobas KRAS test: <10% tumour content; insufficient DNA concentration; Sanger: no PCR amplification; or difficult sequence interpretation; therascreen: positive controls have not amplified specific product, mixed standard delta Ct is not within specified range, no template control has Ct <38, any sample with control gene Ct >34.
It seems that P. ramorum has a larger overall number of recent gene duplications but that particular RXLR effector genes have not been amplified to as high a level as that observed in P. sojae.
Phylogenetic analysis revealed an SWS2 gene duplication event that occurred in the common ancestor of bluefin killifish, A. anableps and guppy, although one of the duplicates had not been amplified from guppy and is reported here for the first time.
When performing BLAST searches with the marker sequences containing the SNP markers that mapped to VI and XII to the P. trichocarpa genome, the best BLAST hits were also on VI and XII (Supplementary Table 1), suggesting that the intended gene fragments had not been amplified.
