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F. noatunensis subsp. orientals Toba04 which is most closely related to F. philomiragia subsp. philomiragia has no IS elements present in its genome.
These observations are in marked contrast to the A. hydrophila genome [ 22], which has no IS elements and only seven pseudogenes.
The recent publication from Sjödin et al., also showed that Francisella noatunensis subspecies has evolved well before Francisella tularensis subspecies and Francisella noatunensis subsp. orientalis has no IS elements and the same scenario might have appeared in those strains as well [ 4].
The group that has no IS 6110 in the NTF region is thought to be the most ancient [ 10].
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The W genome contains 24 IS elements, which is significantly fewer than K-12, B or Crooks; as a consequence, W has no IS-related gene inactivation occurring in the chromosome, whereas K-12 and B both have a number of genes inactivated.
"Ah've no been tae bed yet.
Having no props is fine too.
"There has been no water, no electricity.
There has been no explanation.
There has been no rain.
There has been no fallout".
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