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If the answer has no alignment or mention of how they worked with a team to accomplish the goal, then you've identified your first great employee.
Pis1 has no close homolog of known structure, but gives a weak BLAST hit (E∼0.027) to the CDP-OH phosphotransferase domain of IPCT-DIPPS from Archaeoglobus fulgidus, which contains approximately 200 residues and six transmembrane α-helices (PDB ID 4MND; not shown in figure 5 because the domain structure has no alignment overlap with the sequence introduced by the frameshift) (Nogly et al. 2014).
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However, in practice there is some lack of power in BLAST due to the fragmented nature of the pig shotgun reads (being fragmented even more by the repeatmasking), and we expect that some of the human-mouse alignment has no longer an orthologues region in the pig genome.
We determined the number of bases in each read that aligned with the corresponding contig from the top hit that had no alignment gaps; where multiple hits with equal scores existed, the first hit listed was used.
To this end, we report an overview of recent efforts in LCD technologies devoted to realize more display modes having no alignment layer, faster switching time and low battery consumption.
Additionally, some unigenes that had no alignment hit in the above databases were further estimated by using ESTScan (3.0.3) software (Iseli et al. 1999) to predict their protein coding sequences (CDS).
Echoing subprime, the college bubble even has its own version of the "principal-agent problem," in which the financial interests of the college (which profits from the loan money) have no alignment with the students (who voluntarily drown themselves in debt).
Additional protein-coding gene models from other teleost genomes (medaka and zebrafish) were mapped onto cod genome regions having no alignment with stickleback (Supplementary Note 17 of [ 3]).
If a unigene had no alignment hit (match) in the above databases, ESTScan software [ 16] was used to predict the CDS domain and its direction.
Although misalignments due to indels may be more likely in the vicinity of LCRs, this does not appear to be a factor, because the relationship between substitution and distance to LCR was only slightly weaker (−0.7432, P value ) when only flanking regions that had no alignment gaps were used.
Simply weighting by the probabilities would result in missing data, since some genomic regions have no alignments.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com